Fig. 1.

CBSE supplementation at the diauxic shift extends CLS. Wild-type (wt[empty]) and α-syn overexpressing (wt[α-syn]) cells were grown in minimal medium containing 2% glucose and required supplements in excess. (A) Cell growth was monitored by counting cell number over time and duplication time (Td) of wt and α-syn expressing cells was calculated as ln2/k, where k is the constant rate of exponential growth. In parallel, (B) extracellular concentration of (left) glucose and (right) ethanol were measured in medium samples collected at different time-points. At the diauxic shift (day 0), CBSE (cocoa) was added and (C) survival over time of the indicated strains was assessed by colony-forming capacity on YEPD plates. 72 h after the diauxic shift (day 3) was considered the first age-point, corresponding to 100% survival. (D) Quantification of chronological survival: data referring to the time-points (days) where chronological aging cultures showed 50% (Mean CLS) and 10% (Max CLS) of survival, as well as, survival integral (SI) measured as reported (Murakami and Kaeberlein, 2009). All data refer to mean values determined in three independent experiments with three technical replicates each. Standard deviations (SD) are indicated. ∗p ≤ 0.05 and ∗∗p ≤ 0.01.