Fig. 3.
The effect of the CBSE is independent from caffeine and theobromine. (A) CLS of yeast wt [α-syn] cells in medium containing 2% glucose in the absence (cnt) or presence of 0.2% CBSE, and its contained metabolites and proteins. (B) ROS content of cells treated for 24 h as in (A). (C) CLS of yeast wt [α-syn] cells in medium containing 2% glucose in the absence or presence of 0.2% CBSE, caffeine, theobromine or a combination of the two (25.96 μg/mL caffeine and 105.4 μg/mL theobromine). (D) ROS content of cells treated for 24 h as in (C). Results are reported as the mean ± standard deviation of three independent experiments. ∗p < 0.05.