Fig. 4.

The CBSE inhibits α-syn aggregation. (A) Fluorescence intensity obtained from flow cytometry analysis of aggresomes of yeast wt [α-syn] cells in medium containing 2% glucose in the absence (cnt) or presence of 0.2% CBSE. (B) α-syn aggregation process, followed by ThT fluorescence, in the absence (cnt) or presence of the CBSE (0.1 and 0.025 mg/ml). (C) SPR sensorgrams of CBSE at different concentrations (8 mg/mL, 5.08 mg/mL, 2.54 mg/mL, 1.27 mg/mL, 0.64 mg/mL) display binding toward α-syn on CM5 sensor chip surface. (D) The CBSE shows dose-dependent binding activity to α-syn protein. Results are reported as the mean ± standard deviation of at three independent experiments.