Fig. 6.

The CBSE activates autophagy in yeast. (A) Western analysis using anti-GFP antibody on total extracts from wt[α-syn][Atg8-GFP] cells treated with 0.2% CBSE for 1 and 2 days. Anti-Cdc34 antibody was used as loading control. (B) Quantification of free GFP of three independent experiments performed as in (A). (C) ROS content of atg8Δ[α-syn] cells in medium containing 2% glucose in the absence (cnt) or presence of 0.2% CBSE for 24 h. (D) Fluorescence intensity obtained from flow cytometry analysis of aggresomes of yeast atg8Δ[α-syn] cells in medium containing 2% glucose in the absence (cnt) or presence of 0.2% CBSE for 24 h. Results are reported as the mean ± standard deviation. ∗p < 0.05.