Fig. 7.

The CBSE reduces α-syn toxicity in neuroblastoma cells. (A–B) Western blot analysis using anti-α-syn, anti-phospho-T172-AMPK, anti-AMPKα, anti-vinculin antibodies (A) and anti-p62/SQSTM1 anti-phospho-Ser555-ULK1, anti-ULK1 and anti-vinculin antibodies (B) on protein extracts from SH-SY5Y pTet-SNCA-FLAG cells untreated, treated with doxycycline or treated with doxycycline and 150 μg/mL CBSE for 48 and 72 h. (C) Representative immunofluorescence (60x) images of SH-SY5Y cells treated with doxycycline (Doxy) alone and in combination with 150 μg/ml CBSE (Doxy + Cocoa) for 48 h, immunolabeled with anti α-syn antibody (C), A11 anti-oligomer antibody (D), and Proteostat R dye (E). Nuclei were stained by DAPI (Blue). Histograms represent mean ± standard deviation of cell fluorescence quantified with the ImageJ software.