Figure 3.

Multiscale H&E to IHC image registration. First, a coarse registration step provides an initial alignment of the entire tissue based on the center-of-mass shift between the H&E image and the IHC image. Second, a fine registration step aligns individual nuclei on tiled sections (1024x1024 pixels) of tissue, resulting in a one-to-one matching of nuclei across the H&E channel and the IHC channel. The 2-channel, shift-invariant tiled image data is stored for downstream deep learning model development.