Fig. 1.

Addition of NRs does not affect enterocyte differentiation. (A) Overview of experimental setup. Human small intestinal organoids were differentiated without or with one of the shown nuclear receptor agonists, followed by transcriptomic characterization and immunofluorescence. (B) Representative bright-field images of intestinal organoids before differentiation and after 5 d of differentiation without or with the addition of a nuclear receptor agonist. (Scale bar, 2,000 μm.) (C) Quantitative PCR analysis of selected markers in human small intestinal organoids in expansion (EM) or differentiated in the presence of BMP without (Control) and with specified nuclear receptor agonists for 7 d. Shown is n = 2 with three donors, tested with one-way ANOVA compared to the control for each donor. Error bars = SD, *P < 0.05.