Figure 2.

Sedimentation velocity AUC of Dda, gp32 and the gp32–Dda–dT20 complex. The sedimentation velocity profiles (fringe displacement) were fitted to a continuous sedimentation coefficient distribution model c(s), as well as to a 2D size-and-shape distribution model, c(s,f/f₀). (A) Single c(s) distribution of Dda. (B) Superimposed c(s) distributions of gp32 at three concentrations. (C) Single c(s) distribution of gp32-Dda-dT20. (D) Velocity data of the gp32–Dda–dT20 complex analyzed with the c(s,f/f₀) model and transformed to a c(s,M) contour plot as a heat map with increasing color temperature to maximum fringes/S value in red. The s- and mass-values are listed in Table 2.