Figure 6.
(A) Growth curves of V. cholerae WT (blue) and ΔSI (red) strains in LB at 37°C. Growth parameters including Vmax and the AUC were extracted from growth curves in LB by measuring the optical density at 600 nm (OD600). Values correspond to the measurement of 24 independent colonies. Relative fitness of V. cholerae WT and ΔSI strains compared with E. coli DH5α PcS::gfp was performed in LB by inoculating cells at a ratio of 1:1. Fitness values were determined from 12 independent experiments by flow cytometry. The P-values were calculated by comparing each measure with that of the WT strain using an unpaired t-test. Ns: not significant. (B) Growth curves of V. cholerae WT (blue) and ΔSI (red) strains in LB at 20, 30 and 42°C, respectively. Values correspond to the measurement of 10 independent colonies.