Figure 1.

One-Month-Old Lmna^–/– Mice Exhibit Cardiac Dysfunction

(A) Representative exteriors (scale bar: 2 cm) and cardiac appearances (scale bar: 2 mm) of 1-month-old wild-type (WT) and Lmna^–/– mice. (B) Heart weight (HW)/body weight (BW) (n = 7) and lung weight (LW)/BW (WT, n = 5; Lmna^–/–, n = 4) of WT and Lmna^–/– mice. (C) Representative echocardiography of WT and Lmna^–/– mice. (D) Left ventricular ejection fraction (LVEF), fractional shortening (FS), and heart rate (HR) in WT and Lmna^–/– mice (n = 7). (E) Left ventricular end-systolic volume (LVESV)/BW, left ventricular end-diastolic volume (LVEDV)/BW, left ventricular end-systolic diameter (LVIDs)/BW, and left ventricular end-diastolic diameter (LVIDd)/BW in WT and Lmna^–/– mice (n = 7). (F) Representative hematoxylin and eosin (HE) staining (scale bar: 1 mm) and Masson’s trichrome staining (scale bar: 100 μm) images of the ventricular transverse section of WT and Lmna^–/– mice. (G) Quantification of the percentage of collagen in myocardial tissue of WT and Lmna^–/– mice (n = 5). (H) Kaplan-Meier curves of WT and Lmna^–/– mice showing a median survival time of 36 days for Lmna^–/– mice (n = 8, Breslow generalized Wilcoxon test). Data are presented as mean ± SD. Data in B, D, E (LVIDs/BW, LVIDd/BW), and G were compared by using Student’s t-test, and data in E (LVESV/BW, LVEDV/BW) were compared by using the Mann-Whitney U test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.