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. 2024 Nov 8;25(1):2421584. doi: 10.1080/15384047.2024.2421584

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — (a) Representative Western blot detecting phosphorylation of ATR at threonine 1989 in MIA PaCa-2 cells after treatment with CF10, 5-FU, or GEM. Protein was collected at 5 time points between 8 and 48 hours, with each drug administered at IC50 concentration. Intensity of pATR (top) was normalized to a loading control (β-actin, bottom) and shown as fold increase as compared to vehicle treated (Veh.) cells. (b) Plotted relative pATR expression of three biological replicates in MIA PaCa-2 cells at 48 hours of CF10 (white), 5-FU (blue), or GEM (red) treatment. p values were calculated using an ordinary one-way ANOVA with significance defined as p < .05. *, p < .05; **, p < .01; ***, p < .001; ns, not significant.