Table 2.
Summary
Parse | 10x | |
---|---|---|
Inter-sample variability | High | Negligible |
Intra-sample variability | High | Negligible |
Sensitivity in gene detection | Higher | Lower |
Minimum number of cells to load | 100,000 | 800 |
Minimum number of recovered cells | 200 | 500 |
Pause points | 7 | 7 |
Benchwork | ~ 11 h | ~ 7.5 h |
Maximum number of samples that can be analyzed in one kit with multiplexing | 40 samples with 2500 cells/ sample (1 WT kit) | 16 samples with 2500 cells/ sample (1 kit with 4 reactions) |
Cell type annotation | Difficult | Easier |
Doublet detection | Based on genetic diversity or computational | With hashtags |