Figure 1. Isolation of Hau I-IV from H. aurora. (A) Gel filtration. Sample, crude extract; column, Sephadex G-50 (2.5 × 90 cm); solvent, 0.01 M phosphate buffer (pH 7.0) containing 0.15 M NaCl; volume per fraction, 8 mL. Toxic fractions - fractions 44 to 52 - are indicated by a bar. (B) Reverse-phase HPLC. Sample, toxic fractions obtained by gel filtration; column, TSKgel ODS-120T (0.46 × 25 cm); elution, linear gradient of acetonitrile in 0.1% TFA; flow rate, 1 mL/min. Hau I to Hau IV were eluted in labeled peaks.