Figure 3.

In lens cells, pan-EphA2 is localized to the cell membrane. Longitudinal lens sections from 4-month-old control and ephrin-A5^-/- mice were stained with pan-EphA2 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). Images of the equator region were taken in sequence along similar areas of the lens. Fluorescence signal from pan-EphA2 appeared along the cell membrane within the lens. In anterior epithelial cells, there was increased pan-EphA2 signal at the apical-apical junction (arrowheads) between epithelial cells and fiber cells. In equatorial epithelial cells, pan-EphA2 was enriched at the basal (open arrowheads) membrane between epithelial cells and the lens capsule and at the apical membrane (arrowheads). In cortical lens fiber cells, pan-EphA2 outlined the cell membrane. There are no obvious differences between control and ephrin-A5^-/- sections. Scale bars, 20 μm.