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Canonically activated EphA2-pY589 protein is localized exclusively in epithelial cells. Longitudinal lens sections from 6-week-old, 4-month-old, and 8-month-old ephrin-A5+/+ and ephrin-A5-/- mice were stained with EphA2-pY589 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). The EphA2-pY589 fluorescence highlighted the lens epithelial cells (arrows) across all age-matched control and ephrin-A5-/- lenses. Scale bar, 50 μm.
