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. 2024 Oct 25;16(20):13039–13075. doi: 10.18632/aging.206144

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Copyright: © 2024 Horner et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Non-canonically active EphA2-pS898 protein is enriched in equatorial lens epithelial cells. Longitudinal lens sections from 4-month-old control and ephrin-A5^-/- mice were stained with EphA2-pS898 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). Images of the equator region were taken in sequence along similar areas of the lens. Mature fiber cells with strong EphA2-pS898 cannot be seen in these images of the peripheral fibers. EphA2-pS898 signal was found to weakly stain anterior epithelial cells, and there was increased signal in equatorial epithelial cells (arrows). There are no obvious differences between control and ephrin-A5^-/- lenses. Scale bar, 20 μm.