Table 2.
Primers sequences for qPCR.
| Gene name | Forward primer | Reverse primer |
|---|---|---|
| β-Actin | TCATGAGGTAGTCTGTGAGGTCCC | GCCTCTGGTCGTACCACTGGTATT |
| igf-1 | TGGAGATGTACTGTGCACCTGCCA | TTGTCTGGCTGCTGTGCTGTCCTA |
| igf-2 | CTATTTCAGTAGGCCAACCAGC | GTGCGGGCATTACAGGAATGAC |
| gh | CTCGCTCAGAGGCTCTTCTC | TGTCTCGTGCTTGTCGATGG |
| ghrh | CGGCTGGGAGATGAAAGAGG | GCACTAGCGAAGAGGACTGG |
| cat | TGTGGCTAACTACCAGCGTG | ACTGTTGTAACGGGCCACAT |
| cu/cn-sod | ACTCCATCATTGGCCGAACC | CACCAGCATTGCCTGTCTTC |
| gstα | GCCCATATACCTGGTGGGAG | CCTCTTGATGGCGGGAATCT |
| myod | ACGCCATCAGCTACATCGAG | TCAGACCAGCGTTTGGAGTC |
| myog | GGCTACCAGGACAGGAACTC | GGCTCAGAGTGAGGTGACAG |
| myf5 | AGGTCGAGATCCTACGCAAC | GAGCTGCTCTCTCCAGGTAG |
| myf6 | AGAACACAGTGCCGTCAGTC | GGACTCGCTGGTTCCTTCTC |
| mstn | CAAGTGTTGAGTGTGTGGCTG | GTATCTCTCCTGGCACGCTTG |
Abbreviations: cat, catalase; cu/zn-sod, cu/zn-superoxide dismutase; gh, growth hormone; ghrh, growth hormone-releasing hormone; gstα, glutathione S-transferases α; igf-1, insulin-like growth factor 1; igf-2, insulin-like growth factor 2; mstn, myostatin; myf5, myogenic factor 5; myf6, myogenic factor 6; myod, myogenic differentiation; myog, myogenin.