Figure EV4. Jag1^Ndr/Ndr T cells are not autoimmune as shown by the transfer to Rag1^−/− hosts.

(A) Schematic of the experiment. (B) Survival curve of the Rag1^−/− animals over the course of 8 weeks following transfer with Jag1^+/+ (n = 6) or Jag1^Ndr/Ndr (n = 8) T cells. (C) Relative quantification of Jag1^+/+→Rag1^−/− and Jag1^Ndr/Ndr→Rag1^−/− mouse weight normalized to its original value on day 0 after T cell transfer over 8 weeks (1 = 100% of original weight, mean ± SD, n = 6–10 mice). (D) Representative H&E staining of intestinal sections (duodenum—top, ileum—bottom) performed 8 weeks after T cell transfer. (E) Comparison of the intestinal inflammatory score calculated using the H&E-stained slides of intestinal sections from the Jag1^+/+→Rag1^−/− (n = 4) and Jag1^Ndr/Ndr→Rag1^−/− (n = 5) mice. (F–H) Representative immunofluorescent images of sections from Jag1^+/+→Rag1^−/− (left) and Jag1^Ndr/Ndr→Rag1^−/− (right) mice intestine after DSS, stained for LCK and FOXP3, counterstained with Hoechst (F). Quantification of average LCK⁺ T cell count/Hoechst area (G), and LCK⁺/FOXP3⁺ Treg/LCK⁺ ratio (H) Means ± SD, Statistical analysis was performed by unpaired, two-tailed Student’s t-test.