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. 2024 Oct 2;16(11):2946–2975. doi: 10.1038/s44321-024-00145-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV3 — (A) Schematic of the experiment. E9.5 embryos were harvested and yolk sac (YS) and embryo proper (EP) were processed separately for flow cytometry analysis. (B) Gating strategy for identification of the Erythrocyte progenitors (Eryp), Macrophage progenitors (MFp), Megakaryocyte progenitors (MKp), and Erythro-myeloid progenitors (EMP) after dead cell exclusion. (C, D) Relative proportion of live Eryp, EMP, MFp, and MKp cells from Jag1^Ndr/Ndr (n = 7), Jag1^Ndr/+ (n = 9), and Jag1^+/+ (n = 8) yolk sac (C) and whole E9.5 embryos (D). Graph represents mean ± SD. (E, F) Dot plot of the re-analyzed median scaled ln-normalized mRNA expression of Notch signaling components in human (E) and mouse (F’) thymic cell populations from Park et al, 2020. One-way ANOVA, multiple comparison with Bonferroni method; n.s., no significant difference.