Figure S2.

Molecular characterization of the zebrafish itgav gene and generation of itgav knockout mutant. (A) The zebrafish itgav gene: The genomic structure of the zebrafish itgav gene, comprising 30 exons, is depicted. (B) itgav^−/− mutant generation: CRISPR/Cas9 genome editing was employed to generate an itgav^−/− mutant. The guide RNA targeted exon 4 just before the conserved tryptophan, resulting in a 3-bp deletion and an early stop codon. (C) Molecular validation: HRM analysis corroborated the deletion, and real-time PCR demonstrated downregulation of itgav mRNA, suggesting nonsense-mediated RNA decay.