Table 2.

Relative gene mRNA expression (relative units) of pro-inflammatory cytokines normalized according to G3PDH housekeeping values.

	IL-12
	Leishmania tarentolae						Leishmania infantum			L. tarentolae + L. infantum
	RI-325			LEM-124			IPT1			RI-325 + IPT1			LEM-124 + IPT1
Infection (hours)	min	med	max	min	med	max	min	med	max	min	med	max	min	med	max
4	2.445	3.714ab	5.464	4.377	7.096c	9.781	1.080	2.261a	3.402	4.816	5.905cd	7.708	4.102	4.699bd	5.169
8	1.922	1.908ab	1.923	3.092	4.132c	5.123	0.200	0.474a	0.737	3.325	3.490bc	3.796	3.239	4.643c	5.230
24	0.938	1.066ab	1.210	1.162	1.297ab	1.490	0.165	0.352b	0.495	2.784	3.228c	4.023	1.021	2.146ac	3.259
48	0.907	0.939abc	1.002	0.425	0.495ab	0.567	0.394	0.373b	0.401	3.232	4.225d	5.212	2.321	2.619cd	3.212
72	0.792	0.809a	0.875	0.423	0.616a	0.782	0.123	0.223a	0.312	5.092	5.694b	6.063	2.734	2.959c	3.214
96	0.482	0.580ab	0.685	0.223	0.262ab	0.324	0.002	0.115b	0.320	6.032	6.704c	7.623	1.240	2.104a	3.023

Statistically differences (P < 0.05) between strains on each time point are indicated. Different letters indicate significative differences. Same letters indicate no significative difference.

min: minimum value of gene expression; med: medium value of gene expression; max: maximum value of gene expression.