Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 2;16(11):2882–2917. doi: 10.1038/s44321-024-00148-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV2 — The image panel depicts representative micrographs of the DCN within the cerebellum, stained with Calbindin (green) and Hoechst (blue). The first column shows low magnification images of the DCN and surrounding areas, with labels for the Molecular layer (Mo), Purkinje cell layer (Pc), Granular layer (Gr), and deep cerebellar nuclei (DCN). Scale bar 100 µm. A red box indicates the area of higher magnification within the DCN shown in the second column. The micrographs in the second column demonstrate a clear reduction of calbindin-positive spheroids with both AAV9-CBh-hAP4B1 and AAV9-SYN-hAP4B1 vectors. The third column shows nuclear staining with Hoechst, and the fourth column presents a merge of Calbindin and Hoechst stains. Scale bar 50 µm. The bar graph reveals a larger number of spheroids in untreated and control-treated Ap4b1 KO mice compared to no spheroids in the WT mice. These spheroids are significantly reduced with both CBh (p < 0.0001) and SYN (p < 0.0001) treatment vectors 9 months following treatment. Data are presented as mean ± standard error of the mean (SEM), with n = 3. The data were analysed using one-way ANOVA followed by Dunnett’s multiple comparisons test. Stars indicate p ≤ 0.0001 (****).