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. 2024 Oct 2;16(11):2882–2917. doi: 10.1038/s44321-024-00148-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — Vectors CBh-hAP4B1 and SYN-hAP4B1 were tested, alongside the vector containing the mouse Ap4b1 gene (mAP4B1). Splenocytes that were prepared from WT mice following were assessed through an ELISpot assay for IFN-γ responses to AP4B1 peptides. (A) Representative images of the spot forming detection revealed following the exposure to the negative control (DMSO), the hAP4B1 peptides and the positive control (Concanavalin A). (B) Treated mice did not show any inflammatory response to the peptides. (C) RT-qPCR of total RNA extracted demonstrated elevated hAP4B1 mRNA expression in the brain, liver and spinal cord of treated WT mice with lower expression within the liver. Data are presented as mean ± SEM, n = 3 per group. Data are analysed by a two-way ANOVA with Tukey’s post hoc multiple comparison test. ns = not significant.