Fig. 6. Protection conferred by Ir-Cm (+MDP) is long-lasting in mice.

Four-month evaluation of Ir-Cm (+MDP) vaccine. Two groups of BALB/c mice were vaccinated with Ir-Cm (+MDP)/CpG or only adjuvant control (CpG alone control), (n = 15 mice per group) as described in Fig. 4, and challenged four months after receiving their last immunization. % infected over time (a), overall infectious burden calculated from an AUC analysis (b), and an average number (Log10) of IFU/mL of vaginal swab suspension over time (c) are shown. Groups were compared utilizing a Kruskal–Wallis test or two-way ANOVA with multiple comparisons, respectively. ****p < 0.0001, **p < 0.01, *p < 0.05, ns not significant, LOD limit of detection.