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NBEAL1 decorates the tip of Golgi-connected secretory tubules positive for CTLA4-RUSH-mCherry (arrows). HeLa cells stably transfected with 3xFlag-EGFP-NBEAL1 and transiently transfected with CTLA4-RUSH-mCherry were imaged 12 min after the addition of 50 µM of biotin. Scale bars 10 µm. Playback speed 18x.
