Table 4.
Chemicals, peptides, and recombinant proteins
| Source | Identifier | |
|---|---|---|
| X-tremeGENE 9 DNA Transfection Reagent | Merck | Cat# 6365809001 |
| ANTI-FLAG M2 Affinity gel | Merck | Cat# A2220-5ML |
| Polybrene | Santa Cruz Biotechnology | Cat# sc-134220 |
| LysoTracker Deep Red | Invitrogen | Cat# L12492 |
| Human Transferrin AlexaFluor647 | Jackson ImmunoResearch | Cat# 009-600-050 |
| Glutathione Sepharose 4B | Merck | Cat# GE17-0756-01 |
| GFP-Trap agarose | Proteintech/Chromotek | Cat# gta |
| SUMOstar protease | LifeSensors | Cat# 4110-500UNITS |
| Amylose Resin | New England Biolabs | Cat# E8021L |
| Pierce Cell surface Biotinylation and Isolation kit | Thermo Fisher Scientific | Cat# A44390 |
| DirectPCR lysis reagent | Nordic BioSite | Cat# 250-301-C |
| cOmplete, EDTA-free Protease inhibitor Cocktail | Roche | Cat# 5056489001 |
| Supersignal west dura extended duration substrate | Thermo Fisher Scientific | Cat# 34075 |