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. 2024 Oct 26;29:101314. doi: 10.1016/j.mtbio.2024.101314

Fig. 6.

Fig. 6

Exosomal miR-183-5p derived from F-ADSCs improved angiogenesis and survival rates and alleviated pyroptosis in random-pattern skin flaps. (A)The volcano plot of miRNA expression difference in EXO and FEXO.(B–C) MicroRNAs expression profile showing the expression difference in EXO and FEXO (D) Representative signaling pathways of genes targeted by significantly differential miRNAs according to KEGG analysis. (E) Digital image of RPSFs on D7 in FEXO, FEXO + 3-MA, FEXONC, FEXONC + 3-MA, FEXOanti-miR−185−5p, FEXOanti-miR−185−5p + 3-MA. (F) The LDBF images of random -pattern skin flaps in FEXO, FEXO + 3-MA, FEXONC, FEXONC + 3-MA, FEXOanti-miR−185−5p, FEXOanti-miR−185−5p + 3-MA groups on D7. (G) The expression of angiogenesis-related protein VEGF; pyroptosis-related proteins NLRP3, GSDMD-N and Cleaved Caspase-1(CC1) and autophagy-related proteins LC3B and p62 in FEXO, FEXO + 3-MA, FEXONC, FEXONC + 3-MA, FEXOanti-miR−185−5p, FEXOanti-miR−185−5p + 3-MA groups. The gels have been run under the same experimental conditions, and cropped blots were used here. (H) The immunofluorescence images of CD31/EMCN positive vessels of skin flaps in FEXONC, FEXONC + 3-MA, FEXOanti-miR−185−5p, FEXOanti-miR−185−5p + 3-MA groups. (I) The immunofluorescence images of CD4/Foxp3 Treg cells in skin flaps. (J) The immunofluorescence images of iNOS/CD68 M1 macrophages in skin flaps. ∗ stands for P < 0.05, ∗∗ stands for P < 0.01, ∗∗∗ stands for P < 0.001, ns stands for not significant.