Fig. 7. Crucial roles of MoHTR1 NLS in the regulation of host target gene expression.
A The transcripts abundance of rice target genes of MoHTR1, including OsMYB4. Each strain, including wild type, ΔMohtr1, and ΔMohtr1::MoHTR1ΔNLS, was inoculated onto 4-week-old rice seedlings, and infected rice was collected at 48 hpi. Transcription levels of host target genes of MoHTR1 were quantified using qRT-PCR, and gene abundance was normalized to actin encoding gene expression. B Features of the vectors used for the luciferase assay in rice protoplast. The LUC reporter gene was expressed under the OsMYB4 promoter, MoHTR1 was expressed under the CaMV 35S promoter, and GUS encoding gene was expressed under the ZmUBQ promoter. C Relative LUC activity in MoHTR1 and MoHTR1ΔNLS transfected rice protoplasts. Reporter, effector, and control vectors were transfected into rice protoplasts, and after 5 h, LUC activity was measured by luminometer. Relative LUC activity was calculated by comparing it to transfection with an empty effector vector. Mean ± SD, n = 3 independent RNA samples from infected rice leaves and independently transfected protoplasts, significance was determined by an unpaired two-tailed Student’s t-test (*p < 0.05, **p < 0.01 and ***p < 0.001). Representative data are shown from independently experiments and source data are provided as a Source Data file.