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. 2024 Nov 11;14:27589. doi: 10.1038/s41598-024-73875-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — ASC differentiation into lineages. (A) Microscopic images of ASC at P4 in proliferation medium (control) or differentiation medium (differentiated) for 3 lineages. Adipocytes were stained with Bodipy probe and Hoechst to visualize neutral lipid droplets and nucleus, chondrocytes were stained with Alcian blue to visualize proteoglycans and osteoblasts were stained with either BCIP/NBT or Alizarin Red S to visualize alkaline phosphatase (early differentiation) or mineralization (late differentiation) respectively (original magnification 10x). Bodipy (B) or ARS (C) quantification of control or differentiated ASC according to age category. n = 6 donors were used in each age category. Paired Wilcoxon test was used to test the significance. * P value < 0,05.