Table 7. Multi-class confirmation methods for antibiotic residue analysis in honey - SLE.
| Compounds* | Sample
weight (g) |
Extraction /
Clean-up |
LC conditions | Detection | CCβ or
LOD (μg/kg) |
References | |
|---|---|---|---|---|---|---|---|
| LC Column | Mobile phase | ||||||
|
Lincosamide (1)
Macrolides (2) Nitrofuran (1) Sulfonamides (3) |
0.2 | ACN : H
2O
(50:50, v/v) H 2O |
InfinityLab Poroshell
120 Phenyl Hexyl (3.0 × 100 mm, 2.7 mm) with InfinityLab Poroshell 120 EC-C18 (3.0 × 5 mm, 2.7 mm) guard column |
0.1% FA in H
2O /
MeOH |
HPLC-Q-TOF-MS
(DAD) (ESI +) |
1.1 – 8.4 | ( von Eyken et al., 2019) |
| HCl 2M
H 2O | |||||||
| Quinolones (21) | 1 | McIlvaine
buffer - EDTA (pH 6.0) |
Waters ACQUITY BEH
C18 (2.1 mm × 50 mm, 1.7 μm). |
H
2O; 0.5mM
ammonium formate; 0.005% FA / MeOH; 0.005% FA. |
UHPLC-MS/MS
(ESI +) |
1.21 – 1.39 | ( İsmail Emir et al., 2021) |
ACN – Acetonitrile; DAD – Diode Array Detector; ESI ± - Electrospray in positive mode (+) / Electrospray in negative mode (-); FA – Formic Acid; HPLC-Q-TOF-MS – High Pressure Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry; MeOH – Methanol; UHPLC-MS/MS – Ultra-High Performance Liquid Chromatography tandem Mass Spectrometry.