FIGURE 1.

CRISPR screening revealed that FCGRT and B2M were involved in HAstV infection. (a) Schema of the screening method for cells resistant to HAstV infection. Caco2 cells transduced with a CRISPR‐Cas9 library pool were infected with HAstV4 (MOI = 5) multiple times until almost no surviving cells were visible. The genomic DNA of the surviving cells was purified, and determined for sgRNA sequences via next‐generation sequencing (NGS). (b) NGS analysis revealed knockout genes enriched in HAstV‐resistant cells. The library contained multiple gRNAs for each target gene, and the X‐axis indicates the number of sgRNA detected for each gene via NGS. Y‐axis represents the total read counts per gene. Each dot represents single sgRNA detected by NGS analysis and multiple sgRNAs targeting FCGRT and B2M were observed in the first (blue) and second (pink) rounds.