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. 2024 Oct 7;481(20):1395–1410. doi: 10.1042/BCJ20240114

Figure 1. FLNA-deficient platelets have diminished stress fiber formation compared to control platelets.

Figure 1.

(A) Lysates from floxed (control) FLNA-deficient (platelet-specific KO) platelets were resolved by SDS–PAGE and probed with an anti-FLNA antibody. Beta-actin is shown as a loading control. (B and C) Floxed (control, B) and FLNA-deficient (platelet-specific KO, C) Platelets were allowed to spread on fibrinogen-coated coverslips for 45 min prior to labeling with Alexa-488-phalloidin. Insets: higher magnification images of the floxed (control) and FLNA-deficient (platelet-specific KO) allowed to spread for 45 min on immobilized fibrinogen. (D) Bar graph depicts the quantification of the presence of visible stress fibers in floxed (control, blue bar) and FLNA-deficient (platelet-specific KO, pink bar) platelets. Calculations were based on the mean percentage (%) of stress-fiber positive platelets per field of view. Data are mean ± SEM and represent three independent experiments. *P < 0.05, based on Student's t-test.