Fig. 5.

STAP-2 regulates the advancement of renal fibrosis through HSP27-PI3K/AKT axis. (A-B) siNC vs. siSTAP2 (A) and LPS-siNC vs. LPS-siSTAP2 (B) KEGG pathway enrichment analysis of RNA-seq data. (C-F) WB (C) and quantification of p-HSP27/HSP27 (D), p-PI3K/PI3K (E) and p-AKT/AKT (F) protein expression following overexpression of STAP2 with or without LPS treatment in vitro. n = 3 samples. (G-J) WB (G) and quantification of p-HSP27/HSP27 (H), p-PI3K/PI3K (I) and p-AKT/AKT (J) protein expression in vivo. n = 6 mice. Error bars represent mean ± SEM. The comparable analysis of multiple groups was performed through two-way ANOVA followed by Tukey’s test. *p < 0.05; **p < 0.01