Fig. 6.

VSIG4 blockade impairs C3b induced macrophages polarization. A Uniform manifold approximation and projection (UMAP) of single-cell RNA-seq from human decidual immune cells isolated from patients with RSA and healthy controls. The single-cell RNA sequencing data used in this study were previously published by Guo [9]. B Feature plot showed ICAM1 and VSIG4 expression in the different clusters between Ctrl vs. RSA as defined in (A). C Gene expression levels of VSIG4 and ICAM1 in decidual macrophages from normal pregnancies and RSA patients. D CLSM images showing the expression of VSIG4 protein in decidual macrophages from normal pregnancies and RSA patients. Scale bars = 5 μm. E Statistical histogram of VSIG4 mean fluorescence intensity of each decidual macrophages from normal pregnancies (n = 3) and RSA patients (n = 3). F Gene expression levels of IL1B, IL6 and TNF in macrophages with or without the addition of VSIG4 blocking antibody during co-culture with dNK. G Representative mean fluorescence intensity of CD80 (upper panel) or CD206 (lower panel) in macrophages with or without the addition of VSIG4 blocking antibody during co-culture with dNK. Experiments were repeated for 3 independent times. P values have been determined by two-tailed paired t-test