FIGURE 8.

KCNB1 is expressed in ARH^POMC neurons. (A) Representative confocal image of a WT hypothalamic slice stained with KCNB1 (green) and POMC (red) antibody. Scale bar 1 mm. (B) Magnification of the ARH area in (A). Scale bar 200 μm. (C) Magnifications of the boxed area in (B) showing individual cells expressing KCNB1 (green), POMC (red), and overlap. Cells co‐expressing both proteins are indicated with arrows in the overlap image on the right. Scale bar 25 μm. (D) Representative magnifications of two cells indicated with arrows in (C), co‐expressing KCNB1 and POMC. Note the granular expression of KCNB1, reflecting cluster organization. Scale bar 10 μm.