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. 2024 Oct 31;18(10):e0012626. doi: 10.1371/journal.pntd.0012626

Fig 1. Short photoperiod induces diapause egg production.

Fig 1

(A) Schematic of rearing conditions, developmental timeline, and diapause (DP) egg status. Eggs from the same clutch were common-garden reared under 26°C, 16L:8D conditions. At the pupal stage, animals were separated into three conditions: high temperature, long photoperiod (red); low temperature, long photoperiod (purple); low temperature, short photoperiod (blue). B) Hatching assay to determine DP egg status. % of eggs hatched after exposure to hatching stimulus within 3 weeks of oviposition. Data is shown as mean with SEM (n = 3 replicates; >50 eggs per replicate; one-way ANOVA followed by Tukey’s multiple comparisons test; * = p < 0.05, ** = p < 0.01). C and D) Egg width (C) and length (D) measurement. Data is shown as median with range (n = 2 replicate experiments; 130–190 eggs per replicate; one-way ANOVA followed by Tukey’s multiple comparisons test; *** = p < 0.001, **** = p < 0.0001). Created with Biorender.com.