Figure 5.

Flow cytometric analysis, a precise and reliable method, was used to assess apoptosis using Annexin V/PI staining and FlowJo software in the MDA-MB-231 cells (A) Representative images of apoptosis in non-irradiated and (B) irradiated groups. This analysis shows the percentage of cells that have undergone apoptotic death and necrosis. (C) The graph illustrates the levels of apoptosis in the experimental groups under both non-irradiated and irradiated conditions. The comparison of these levels, which is of significant importance, underscores the synergistic effects of irradiation and PI3Kca gene knockout on apoptosis (*P<0.05; ** P<0.01; ***P<0.001; and **** P<0.0001); NC: Negative control; SCR: Scramble, and Ko-PI3: PI3Kca gene knock-out