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. 2024 Sep 19;137(21):2644–2646. doi: 10.1097/CM9.0000000000003274

Figure 1.

Figure 1

DcR3 suppresses the NF-κB pathway and the NLRP3 inflammasome activation in gouty inflammation. (A) Expression of DcR3, TL1A, and DR3 mRNA levels in gout patients’ PBMCs. (B) Expression of DcR3, TL1A, and DR3 protein levels in gout patients’ PBMCs. (C) Expression of DcR3 protein concentration in gout patients’ peripheral blood plasma. (D) Relative expression of DcR3, TL1A, and DR3 mRNA levels in PBMCs between the patients with gout flares and after resolution. (E) Relative expression of DcR3 protein concentration in peripheral blood plasma between the patients with gout flares and after resolution. (F) Relative expression of DcR3, TL1A, and DR3 protein levels in PBMCs between the patients with gout flare and after resolution. (G) Effect of DcR3 on the mRNA levels of IL-1β, TNF-α, TGF-β1, IL-10, TL1A, and DR3 in THP-1 cells treated with MSU. (H) Effect of DcR3 on the production of IL-1β, TNF-α, TGF-β1, and IL-10 in the supernatant of THP-1 cells treated with MSU. (I) Effect of DcR3 on the protein levels of IL-1β, TL1A, and DR3 in THP-1 cells treated with MSU. (J) Effect of DcR3 on the mRNA levels of IκBα, NF-κB, NLRP3, ASC, and caspase-1 in THP-1 cells treated with MSU. (K, L) Effect of DcR3 on the protein levels of p-IκBα, p-NF-κB, NLRP3, ASC, pro-caspase-1, and cleaved caspase-1 in THP-1 cells treated with MSU. *P <0.05; †P <0.01; ‡P <0.001; ns, no statistic significance. AG: Acute gout; ASC: Apoptosis-associated speck-like protein; DcR3: Decoy receptor 3; DR3: Death receptor 3; HC: Healthy controls; IG: Intercritical gout; IκBα: Inhibitor kappa B alpha; IL-10: Interleukin-10; IL-1β: Interleukin-1β; MSU: Monosodium urate; NF-κB: Nuclear factor kappa-B; NLRP3: Nucleotide-binding oligomerization domain receptor protein 3; PBMCs: Peripheral blood mononuclear cells; pcDNA-DcR3: Overexpression plasmid of DcR3; p-IκBα: Phosphorylated inhibitor kappa B alpha; p-NF-κB: Phosphorylated nuclear factor kappa-B; si-DcR3: DcR3-specific small interfering RNA; TGF-β1: Transforming growth factor-β1; TNF-α: Tumor necrosis factor; TL1A: Human tumor necrosis factor-related ligand 1A.