Figure 1:

altered cell composition in the inflamed joint of JIA. Synovial fluid mononuclear cells (SFMC, n = 18) and PBMC of JIA patients (n = 52) and healthy controls (HC, n = 18) were stained and acquired on a full-spectrum cytometer. Unbiased clustering algorithm FlowSOM, after gating on all single, live (FVD−) cells, identified 18 clusters of major cellular compositions with UMAP of all samples in (A). (B) Heatmap of the 32 markers used for clustering, MFI Z-score across columns. (C) Expression UMAP of lineage markers CD3, CD4, CD19, CD56, CD123, and CD11c and validation of the cluster identity. (D) 18 clusters UMAP for HC PBMC, JIA PBMC, and JIA SFMC. (E) Major immune cell population (as defined in C) frequencies of normalized UMAP of all samples per sample group in HC PBMC, JIA PBMC, and JIA PBMC