FIGURE 4.

Evening blue light for 3 h acutely reduces sleep in CS flies and increases nighttime sleep both CS and w¹¹¹⁸. (A) Timeline of entire experiment, showing 2 d of baseline 12:12 WD, 6 d experimental 9:3:12 white/blue/dark (WBD), 2 d recovery WD. (B) Sleep patterns for male and female CS (red) and w¹¹¹⁸ (gray) flies. Sleep (min) during each 30-min bin of the day was averaged across flies for each group and plotted to show overall sleep profiles over the second baseline and first four experimental days. (C) Differences in sleep between experimental days and baseline of male and female CS and w¹¹¹⁸. Mean sleep duration (min) in each 30-min bin of the baseline day was subtracted from the mean sleep duration in each respective bin on the first four experimental days. (D) Total sleep duration (min) during the 3-h blue light exposure (ZT9–12) and the 3 h immediately after (ZT12–15). Sexes and genotypes were analyzed separately with a repeated-measures ANOVA or nonparametric alternative, followed by post-hoc tests. Asterisks indicates significant difference in sleep during 3-h bin (per post-hoc test) within group between experimental/recovery and baseline day (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Ns were 31, 25, 29, 29 for CS female, w¹¹¹⁸ female, CS male, w¹¹¹⁸ male, respectively.