Figure 2.

Ectopic expression of PanNSP2 in Parasponia enhances mycorrhizal colonization. Mycorrhizal colonization grown at low exogenous phosphate condition (20 µM PO₄ ^3-) (A–C) and high exogenous phosphate condition (3000 µM PO₄ ^3-) (D–F). Parasponia lines 1 to 3 are transformed with pLjUBQ1:mPanNSP2intron construct, whereas lines 4 to 6 NSP2 contain pLjUBQ1:mPanNSP2. Different letters indicate significant differences (p<0.05) between these lines as determined by One-way ANOVA followed by a Tukey’s post hoc test. Plants were harvested 6 weeks post-inoculation (6 wpi) with 300 spores/plant. All data are displayed in box plots, showing data points, the median, and the interquartile range (IQR).