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. 2024 Nov 13;38(1):18. doi: 10.1007/s13577-024-01147-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Self-renewal marker expression of hiPSC lines derived from cystic BA patients. A Morphologies of BA-specific hiPSCs taken with phase contrast microscopy. Scale bars, 200 µm. B Immunocytochemistry of OCT3/4 and NANOG on cBA-hiPSCs. Scale bars, 200 µm. C Flow cytometry analysis of SSEA-4 and TRA-1-60 on cBA-hiPSCs. Top panels: samples with anti-SSEA1 and anti-TRA-1-60 antibodies. Bottom panels: samples with control IgG. D The ratio of positive cells for SSEA-4 and TRA-1-60. The bar graph shows mean + standard errors (SE), n = 3 (biological samples)