Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 12;224(1):e202406103. doi: 10.1083/jcb.202406103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 MRC Laboratory of Molecular Biology

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 5. — Cab45-SURF4 interaction is SP cleavage-dependent and occurs co-translationally. (A) Proteinase K (PK) protection assay of Cab45-T44* or Cab45 with no amber suppression, treated with an increasing concentration of cavinafungin (CVF; 1 μM, 10 μM in DMSO [1% of IVT reaction volume]) or vehicle DMSO. Samples were taken from total lVT reactions. Positions of different species of Cab45 are indicated. (B) The same samples as in A but Cab45-HA and FLAG-SURF4 IPs were performed on UV-crosslinked semi-permeabilized cells. Green arrow indicates Cab45-SURF4 crosslink. (C) Cab45 C-terminal truncations were translated in vitro and photo-crosslinked to FLAG-SURF4. Following UV-crosslinking and FLAG-IP, cross-linked products were resolved by SDS-PAGE. * indicates a consistent cross-link that was later identified to be TRAPα (Fig. S4 G). Blue * indicates an unidentified crosslinked species. Source data are available for this figure: SourceData F5.