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. 2024 Nov 13;14:27768. doi: 10.1038/s41598-024-75142-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — PKR interacts with 4.1R depending on its kinase activity. (A) COS7 cells were transfected with the indicated plasmids, followed by IP and WB. The top panel shows the co-precipitation of PKR with 4.1R by IP with anti-Flag antibody and WB with anti-Myc antibody. The second panel shows the expression of 4.1R by IP with anti-Flag antibody and WB with anti-Flag antibody. The lower two panels show the expression level of each protein without IP. (B) COS7 cells were transfected with the indicated plasmids, followed by IP and WB. The top panel shows the co-precipitation of 4.1R with PKR by IP with anti-Flag antibody and WB with anti-Myc antibody. The second panel shows the expression of PKR by IP with anti-Flag antibody and WB with anti-Flag antibody. The lower two panels show the expression level of each protein without IP. (C) COS7 cells were transfected with the indicated plasmids, followed by IP and WB. The top panel shows the co-precipitation of wild type PKR or PKR^K296R with 4.1R by IP with anti-Flag antibody and WB with anti-Myc antibody. The second panel shows the expression of 4.1R by IP with anti-Flag antibody and WB with anti-Flag antibody. The lower two panels show the expression level of each protein without IP.