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. 2024 Nov 12;15:9797. doi: 10.1038/s41467-024-54136-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Changes in median lifespan in wildtype and hsf-1 overexpressing (hsf-1 OE) worms after RNAi against HSF-1 target genes (sample numbers for all groups can be found within Supplementary Data 1). b, c STRING networks of screened genes that, when knocked down, were found to (b) decrease hsf-1 OE lifespan by > 20% with no effect on wild-type lifespan, or (c) increase hsf-1 OE lifespan by > 20% but decrease wildtype lifespan by the same degree. Black lines represent interaction by co-expression, and purple lines represent evidence of experimental interaction. ubql-1 was included in (c) for reference, and genes without any connections were removed. d Relative expression of ubql-1 mRNA on day 1 of adulthood in wildtype, ubql-1(tm1574), hsf-1 OE, and hsf-1 OE; ubql-1(tm1574) animals grown on OP50. Values were normalised to the geometric mean of the housekeeping genes rpb-2, pmp-3, and cdc-42. Data plotted are the mean of 4 biological replicates +/− SD (wildtype vs ubql-1(tm1574), p = 0.0008; hsf-1OE vs hsf-1OE;ubql-1(tm1574), p < 0.0001; wildtype vs hsf-1OE, p = 0.0005; ubql-1(tm1574) vs hsf-1OE;ubql-1(tm1574), p = 0.8662). e, f Lifespan of (e) wildtype and hsf-1 OE animals on empty vector and ubql-1(RNAi) (wildtype vs ubql-1(RNAi), p = 0.2886; wildtype vs hsf-1OE, p < 0.0001; hsf-1OE vs hsf-1OE;ubql-1(RNAi), p = 0.0002) and (f) wildtype, ubql-1(tm1574), hsf-1 OE, hsf-1 OE;ubql-1(tm1574) animals grown on OP50 (wildtype vs ubql-1(tm1574), p = 0.0002; wildtype vs hsf-1OE, p < 0.0001; hsf-1OE vs hsf-1OE;ubql-1(tm1574), p < 0.0001). Statistical significance was calculated using (d) two-way ANOVA with Fishers LSD and (e, f) Mantel-Cox log-rank test. ns, not significant (p > 0.05), **p < 0.01, ***p < 0.001, ****p < 0.0001. Full statistics for lifespan trials (including n values) can be found in Supplementary Data 2. Source data are provided as a Source Data file.