TABLE 5.
Results of the bacterial reverse mutation assay with Organic Cordyceps M2-116-04 powder.
| Revertant colonies per plate | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Concentration (µg/plate) | Salmonella typhimurium | Escherichia coli | ||||||||
| TA98 | TA100 | TA1535 | TA97a | WP2 uvrA | ||||||
| -S9 | +S9 (5%) | -S9 | +S9 (5%) | -S9 | +S9 (5%) | -S9 | +S9 (5%) | -S9 | +S9 (5%) | |
| 50 | 27.5 ± 5.2 | 32.3 ± 8.5 | 138 ± 4.28 | 164 ± 7.0 | 12.7 ± 4.5 | 14.3 ± 4.8 | 118 ± 2.5 | 115 ± 3.5 | 61.2 ± 3.5 | 60.3 ± 10.0 |
| 100 | 33.4 ± 4.3 | 36.5 ± 4.8 | 139 ± 4.6 | 162 ± 2.1 | 13.3 ± 3.5 | 156 ± 4.3 | 122 ± 4.1 | 123 ± 4.8 | 65.5 ± 6.6 | 61.3 ± 8.5 |
| 500 | 34.5 ± 3.5 | 35.5 ± 4.7 | 141 ± 4.5 | 158 ± 7.5 | 14.2 ± 3.5 | 16.4 ± 4.9 | 122 ± 4.6 | 127 ± 4.8 | 67.4 ± 3.3 | 66.3 ± 7.6 |
| 1,000 | 36.7 ± 4.0 | 37.8 ± 4.5 | 145 ± 4.6 | 163 ± 12.5 | 14.9 ± 4.5 | 18.4 ± 5.8 | 124 ± 4.4 | 127 ± 4.4 | 68.3 ± 5.0 | 67.7 ± 7.6 |
| 5,000 | 37.7 ± 5.5 | 39.3 ± 5.5 | 146 ± 5.6 | 176 ± 9.0 | 15.3 ± 4.9 | 18.2 ± 4.7 | 227 ± 4.2 | 120 ± 4.2 | 70.4 ± 5.6 | 69.7 ± 5.7 |
| Positive control | 1706 ± 201* | 1,363 ± 201* | 2,887 ± 150* | 2,867 ± 150* | 2,163 ± 403* | 2,363 ± 403* | 1,298 ± 135* | 1,398 ± 135* | 1,325 ± 125* | 1,425 ± 125* |
| Negative control | 33.4 ± 3.4 | 33.6 ± 3.4 | 134 ± 21.0 | 154 ± 21.0 | 11.4 ± 2.5 | 155 ± 2.5 | 129 ± 23.1 | 169 ± 23.1 | 72.3 ± 14.5 | 777 ± 14.5 |
Data shown as mean ± SD.
*Statistical significance compared to control, p < 0.05.
-S9 positive controls for Strain 98: 2-nitrofluorene (2NF); Strains TA, 97a, TA, 100; TA, 1535: Sodium-azide (NaN3); Strain WP2: methyl-methanesulfonate (MMS).
+S9 (5%) positive controls for Strain 98: Benzo(a)pyrene(BP); Strains TA, 97a, TA, 100; TA, 1535, WP2: 2-aminoanthracene (2AAn).