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. Author manuscript; available in PMC: 2024 Nov 13.
Published in final edited form as: Immunology. 2022 Mar 31;166(3):310–326. doi: 10.1111/imm.13473

FIGURE 3.

FIGURE 3

Analysis of signalling pathways induced by IL-4 during the Tc17 reprogramming. (a) Activation of Stat signalling. (b) Activation of Akt, ERK, P38, and JNK. IL-2-, IL-4-, or IL-7-triggered Stat, Akt, and MAPK signalling in polarized Tc17 cells. Tc17 cells were treated with designated cytokines for 15 min, then analysed by Western blotting. Actin was used as loading control. (c) Western blot analysis of Tc17 cells following siRNA knockdown of Stat2 and Stat6. (d) Representative contour plots show IL-4-induced Tc17 conversion to IFN-γ-producing cells in Stat2- and Stat6-cells following siRNA knockdown. (e) Specific inhibition of IL-4-induced AKT signalling in Tc17 cells using MK2206. (f) MK2206 suppresses IL-4-induced Tc17 reprogramming. DMSO was used as solvent control. Percentage of IL-17A−/IFN-γ+ cells after IL-4 treatment was shown at right. ****P < 0.0001. All experiments were independently repeated three times with similar results