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. Author manuscript; available in PMC: 2024 Nov 13.
Published in final edited form as: Immunology. 2022 Mar 31;166(3):310–326. doi: 10.1111/imm.13473

FIGURE 4.

FIGURE 4

Eomes regulates IL-4-induced Tc17 reprogramming. (a) qPCR of mouse Tbx21 (T-bet), Rorc (RORγt), Gata3, and Eomes in naïve CD8 cells and polarized Tc1, Tc17, and IL-4-treated Tc17 cells. Relative fold changes to naïve CD8 cells are shown. ***P < 0.001; n.s.: not significance. (b) Inhibition of PI3K/AKT/mTOR pathway suppressed IL-4-induced Eomes expression in Tc17 cells. Polarized Tc17 were treated with or without IL-4 and with indicated compounds (1.0 μM each). Cumulative flow cytometry data showing percentage of Eomes positive cells in CD8+ T cells is shown. (c) Conditional knockout of Eomes in Tc cells hampers IL-4 triggered Tc17 reprogramming. Contour plots and cumulative flow cytometry data show loss of Tc17 reprogramming upon Eomes ablation. **P < 0.01. These experiments were independently repeated three times with similar results