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. Author manuscript; available in PMC: 2024 Nov 13.
Published in final edited form as: Immunology. 2022 Mar 31;166(3):310–326. doi: 10.1111/imm.13473

FIGURE 6.

FIGURE 6

Ex vivo culture protocol for transforming Tc17 from human PBMC. (a) Procedure of polarization human PBMC into Tc17 and secondary IL-4 stimulation. (b) Frequency of human IL-17A and IFN-γ producing Tc cells with or without IL-4 stimulation from healthy donors. Representative graphs are shown at left. Mean ± SD was shown at right (n = 5). (c) Frequency of human IL-17A and IFN-γ producing Tc cells with or without IL-4 stimulation from PBMC of colorectal cancer patients. Representative graphs were shown at upper panel. Cell population changes of each patient are shown at lower panel (n = 10). **P < 0.01, ***P < 0.001