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. 2024 Oct 22;10(11):7155–7166. doi: 10.1021/acsbiomaterials.4c01226

Figure 3.

Figure 3

Response of the melanoma cytoskeleton to the drug treatment. (A) WM35 and (B) WM266-4 melanoma cells after 24 h of treatment with colchicine, colchicine + Y27632, and colchicine + blebbistatin. Actin filaments (F-actin, Alexa Fluor 488), microtubules (β tubulin, Alexa Fluor 555), and cell nuclei (Hoechst 34580). (C) Changes in mechanical properties of drug-treated (24 h) WM35 (RGP) and (D) WM266-4 (skin metastasis) melanoma cells measured using AFM. Apparent Young’s modulus was calculated for a load force of 2 nN (resulting in an indentation depth of 500–1000 nm).